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 Rare Mutation Detection Best Practices Guidelines - Bio-Rad
Bio-Rad Laboratories offers validated RMD assays for cancer mutations. Each pair of mutation and reference assays contains a single set of primers and two competitive probes, one detecting the WT allele (in HEX), and one detecting the mutant allele (in FAM) (Figure 1A). When running a mixed sample with PrimePCR ddPCR Mutation Detection Assays, the
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